Available Technology
Simultaneous study of multiple genetic interactions using multi-mutant yeast
Markets Addressed
Yeast strains can be engineered for deficient phenotypes (e.g. missing drug efflux pumps), which can be useful for studying drug mechanisms of action. Other applications include rapid discovery of synthetic lethality interactions and identification of new biochemical pathways and pathway members.
Innovations and Advantages
While the Saccharomyces Genome Deletion Project succeeded in deleting many open reading frames in the S. cerevisiae genome, the possibility of multiple deletions in the same strain still represents an unmet challenge. A method has been discovered to precisely delete large numbers of genes in a single S. cerevisiae strain. Current methods have produced strains with approximately 20 engineered deletions. However, these methods also induce undesirable genome rearrangement. The invention may efficiently produce strains deleted for hundreds of genes, with fewer rearrangement side effects. mutated yeast offspring.
Additional Information
Intellectual Property Status: Patent(s) pending
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Inventor(s):
King, Oliver D.
Roth, Frederick P.
Zhang, Lan V.
Categories:
For further information, please contact:
Grant Zimmermann, Director of Business Development
(617) 495-3067
Reference Harvard Case #2406