Available Technology
A tool for the linkage and presentation of multiple protein functional domains
Technology:
Novel approach allows investigators to quickly generate platforms for the multivalent display of protein domains
Markets Addressed
Ligand Presentation Framework - a system that enables the presentation of multiple copies of the same or a mixture of ligands in a conformationally constrained structure.
Multimeric solid phase linker. a system to immobilize multiple ligands to solid phases for signal amplification, purification etc.
Cell surface receptor studies. study cell surface receptors, cellular interactions, cell surface signaling etc.
Process chemistry, link two or more enzymes in linked partway.
Innovations and Advantages
Recent molecular biology research is moving beyond genetics to look at protein-protein interactions and how cells interact, or communicate. These exciting areas are in need of improved tools that allow these interactions to be identified and studied.
Harvard Medical School researchers have developed a novel approach that allows investigators to quickly generate platforms for the multivalent display of protein domains. By the use of a cassette expression system, researchers can quickly insert active protein domains (enzymes, receptors, receptor ligands, antigens, etc.) into either the amino or carboxy terminus of a protein that forms a rigid octameric scaffold. An extension of the system provides the capability of covalently linking the hybrid peptides to inert support matrices, other peptides, oligonucleotides, or other macromolecules such as nucleic acids.
The clear advantages are that it makes for easy construction of protein hybrids and is multimeric.
Additional Information
Intellectual Property Status: Issued U.S. patent nos.: 6,844,171
Tweet
Inventor(s):
Elkin, Carl
Filman, David
Hogle, James M.
Zuccola, Harmon
Categories:
For further information, please contact:
Grant Zimmermann, Director of Business Development
(617) 495-3067
Reference Harvard Case #1528