Available Technology
Monoclonal antibody 2F12, Anti-ITK
Technology:
Monoclonal antibodies
Markets Addressed
Applications include: Western blotting, immunoprecipitation, and immune complex kinase assays, as well as the detection of the signaling protein ITK (tyrosine kinase).
Innovations and Advantages
Monoclonal antibody to the T-cell signaling protein ITK (tyrosine kinase) has been developed by Heather Wilcox in Dr. Leslie Berg's lab, in the department of Molecular and Cell Biology at Harvard. These monoclonal antibodies were raised against a GST fusion protein comprising the first 26 amino acids of murine ITK. The clone is 2F12, an IgG1, and it works for immunoprecipitation and Western blot. It apparently works for intracellular staining. It recognizes a single protein of 72kDa. The clone has been grown as ascites. There is cross-reactivity to human ITK, and possible cross-reactivity to species that show homology in the first 26 amino acids of the protein.
Additional Information
Publication:
Heyeck SD, Wilcox HM, Bunnell SC, Berg LJ. 1997. Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity. J Biol Chem. 272(40):25401-8.
Tweet
Inventor(s):
Heyeck, Stephanie Dumas
Wilcox, Heather M.
Categories:
For further information, please contact:
Debra Peattie, Director of Business Development
(617) 495-3067
Reference Harvard Case #1490