A rapid and inducible method for repressing gene function
Various systems have been developed for the directed inactivation of a targeted gene in a eukaryotic cell, however the study of genes essential for the viability of the cell remains difficult. The Cre/lox system allows for the inducible deletion of the targeted gene, but the remaining mRNA and polypeptide products of the gene may only be slowly titrated out of the host cell.
Innovations and Advantages
The Struhl lab has developed a rapid and effective means to inactivate a target gene, including genes involved in essential pathways. This invention allows the inducible degradation of the polypeptide product in addition to the repression of a targeted gene. In combination, this double shut-off provides for a thorough block to continued target gene function.
Intellectual Property Status: Harvard's intellectual property portfolio includes the following patent:
US 6,576,469 issued June 10, 2003.
Z. Moqtaderi, Y. Bai, D. Poon, P.A. Weil, K. Struhl, Nature 1996, 383, 188-191.
For further information, please contact:
Grant Zimmermann, Director of Business Development
Reference Harvard Case #1426